VHH Screening Service | Antibody production

　　About VHH Screening　　　　　　　　　　　　　　　　　

This is a contract service for antibody screening using VHH-presenting phage synthetic libraries in Japan.

No royalties are required for the gene sequence information provided, and no animals are used in the testing.

We provide a packaged screening service at low cost and speed.

　　About VHH antibody　　　　　　　　　　　　　　　　　　　

In addition to IgG antibodies consisting of H and L chains, alpacas and other camelids have heavy chain antibodies consisting only of H chains, and their antibody variable regions are called VHH antibodies.

This VHH antibody has the following characteristics.

・Molecular weight 1.3kDa~1.5kDa, excellent refolding after denaturation

・High stability against temperature, pH, denaturants and other environmental factors

・Easy to modify by protein engineering such as multivalency and compound modification

・Has the same affinity as normal antibodies

・It is relatively easy to adjust mass production using E. coli.

With this background, VHH antibodies are expected to be utilized in various fields including drug discovery research.

The "VHH Screening Service" allows you to quickly search for antibodies that react specifically to a target by screening using a VHH-presenting phage synthetic library.

　　VHH Screening Service Package　　　　　　　　　　　　

☑Test Outline

【STEP1】

1. Antigen donation

　Please provide biotin-modified antigens (100 μg or more).

　Biotin modification is also available as an option.

2. Biopanning

　After immobilization of the antigen, biopanning is performed using a VHH antibody-presenting phage library.

3. Binding and specificity evaluation

　Assesses the binding of VHH antibody-presenting phage obtained by biopanning to antigen.

【STEP2】

1. Sequence